Year : 2019 Volume : 49 Issue : 1

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Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts

Open Access

Abstract

The methanol extracts from different parts of Rheum ribes were subjected to qualitative and quantitative phytochemical and in vitro biological activity (antioxidant, anti-urease, anticholinesterase). Qualitative phytochemical tests were performed using standard analysis methods and these studies revealed the presence of phenolics and tannins. Following this, a quantitative determination of total phenolics and tannins contents was carried out. The antioxidant activity of the extracts were assayed using DPPH, FRAP, TEAC/ABTS and CUPRAC techniques. In addition, the anti-urease and anticholinesterase activity of the extracts were examined using indophenol and Ellman methods, respectively. In this study, it was determined that the macerated flowers extract contained higher total phenolic and tannins contents than the other extracts. According to the results obtained from the antioxidant experiment, the macerated extract of flowers showed the strongest ABTS.+ scavenging and ferric reducing antioxidant power activity. The macerated leaves and Soxhlet radix extracts exhibited the strongest DPPH. scavenging and cupric reducing antioxidant activity, respectively. The young shoots extracts obtained using the Soxhlet methods showed the highest anticholinesterase activity. All extracts obtained from different parts of the plant were found to have very low anti-urease activity when compared to the anti-urease activity of standard compound. Therefore, methanol extracts from plant’s flowers, leaves and young shoots can be used as a natural antioxidant and anticholinesterase agent respectively, for the pharmaceutical and food industry in the future. Cite this article as: Taşkın T, Bulut G (2019). Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts. Istanbul J Pharm 49 (1): 7-13.

Keywords

Rheum-ribes   antioxidant   anti-uresae   anticholinesterase   phytochemical-analysis  

Corresponding Author

Turgut Taşkın

References

  • Amin M, Anwar F, Naz F, Mehmood T, Saari N (2013). Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 18: 2135-2149.
  • Amiri N, Shafaghat A, Salimi F (2015). Screening of the essential oil, hexane extract, chemical composition, antioxidant activity, and antimicrobial activity of the flower Rheum ribes L. from Iran. J Essent Oil Bear Pl 18: 1108-1115.
  • Andiç S, Tunçtürk Y, Ocak E, Köse S (2009). Some chemical characteristics of edible wild rhubarb species (Rheum ribes L.) Res. J Agric Biol Sci 5: 973-977.
  • Baskar R, Shrisakthi S, Sathyapriya B, Shyampriya R, Nithya R, Poongodi P (2011). Antioxidant potential of peel extracts of banana varieties (Musa sapientum). Food Nutr Sci 2: 1128-1133.
  • Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239: 70-76.
  • Bulut G, Biçer M, Tuzlacı E (2016). The folk medici
Daha Fazla Göster

Details

DOI 10.26650/IstanbulJPharm.2019.18012

Full Text (PDF)

Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts

Open Access

Abstract

The methanol extracts from different parts of Rheum ribes were subjected to qualitative and quantitative phytochemical and in vitro biological activity (antioxidant, anti-urease, anticholinesterase). Qualitative phytochemical tests were performed using standard analysis methods and these studies revealed the presence of phenolics and tannins. Following this, a quantitative determination of total phenolics and tannins contents was carried out. The antioxidant activity of the extracts were assayed using DPPH, FRAP, TEAC/ABTS and CUPRAC techniques. In addition, the anti-urease and anticholinesterase activity of the extracts were examined using indophenol and Ellman methods, respectively. In this study, it was determined that the macerated flowers extract contained higher total phenolic and tannins contents than the other extracts. According to the results obtained from the antioxidant experiment, the macerated extract of flowers showed the strongest ABTS.+ scavenging and ferric reducing antioxidant power activity. The macerated leaves and Soxhlet radix extracts exhibited the strongest DPPH. scavenging and cupric reducing antioxidant activity, respectively. The young shoots extracts obtained using the Soxhlet methods showed the highest anticholinesterase activity. All extracts obtained from different parts of the plant were found to have very low anti-urease activity when compared to the anti-urease activity of standard compound. Therefore, methanol extracts from plant’s flowers, leaves and young shoots can be used as a natural antioxidant and anticholinesterase agent respectively, for the pharmaceutical and food industry in the future. Cite this article as: Taşkın T, Bulut G (2019). Qualitative and quantitative phytochemical analysis and in-vitro biological activity of Rheum ribes L. different parts. Istanbul J Pharm 49 (1): 7-13.

Keywords

Rheum-ribes   antioxidant   anti-uresae   anticholinesterase   phytochemical-analysis  

Corresponding Author

Turgut Taşkın

References

  • Amin M, Anwar F, Naz F, Mehmood T, Saari N (2013). Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 18: 2135-2149.
  • Amiri N, Shafaghat A, Salimi F (2015). Screening of the essential oil, hexane extract, chemical composition, antioxidant activity, and antimicrobial activity of the flower Rheum ribes L. from Iran. J Essent Oil Bear Pl 18: 1108-1115.
  • Andiç S, Tunçtürk Y, Ocak E, Köse S (2009). Some chemical characteristics of edible wild rhubarb species (Rheum ribes L.) Res. J Agric Biol Sci 5: 973-977.
  • Baskar R, Shrisakthi S, Sathyapriya B, Shyampriya R, Nithya R, Poongodi P (2011). Antioxidant potential of peel extracts of banana varieties (Musa sapientum). Food Nutr Sci 2: 1128-1133.
  • Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239: 70-76.
  • Bulut G, Biçer M, Tuzlacı E (2016). The folk medici
Daha Fazla Göster

Details

DOI 10.26650/IstanbulJPharm.2019.18012

Full Text (PDF)